Enhanced isolation of Legionella species from composted material

AbstractLegionella pneumophila and Legionella species were isolated from composted material when freshly prepared buffered charcoal yeast extraction (BCYE) was supplemented with glycine (1.5 g/L), polymyxin B sulfate (40000 IU/L), vancomycin hydrochloride (0.5 mg/L) and cycloheximide (40 mg/L) (GVPC medium) and Modified Wadowsky-Yee MWY (Oxoid ™, Cambridge, England) plates were used for cultivation but not with commercially sourced pre-poured GVPC and MWY plates (Oxoid™, Cambridge, England). L. cincinnatiensis and pathogenic L. pneumophila Sg 1 Benidorm and France/Allentown were identified as well as a non-typeable (NT) strain of L. pneumophila. As most laboratories no longer produce their own media, this may contribute to the lack of positive cultures from composted material. Th...

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