Plasma‐activated carbon nanotube‐based high sensitivity immunosensors for monitoring Legionella pneumophila by direct detection of maltose binding protein peptidoglycan‐associated lipoprotein (MBP‐PAL)

Live Legionella pneumophila induces MUC5AC production by airway epithelial cells independently of intracellular invasion

Yoshitomo Morinaga,a Katsunori Yanagihara,a Nobuko Araki,a Yohei Migiyama,a,b Kentaro Nagaoka,a,b Yosuke Harada,a,b Koichi Yamada,a,b Hiroo Hasegawa,a Tomoya Nishino,b Koichi Izumikawa,b Hiroshi Kakeya,b Yoshihiro Yamamoto,b Shigeru Kohno,b,c Shimeru Kamihiraa aDepartment of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki, 852-8501, Japan.

bSecond Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

cGlobal COE Program, Nagasaki University, Nagasaki, Japan.

Published on the web 20 January 2012.

Canadian Journal of Microbiology, 2012, 58:(2) 151-157, 10.1139/w11-123

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Azithromycin inhibits MUC5AC production induced by the Pseudomonas aeruginosa autoinducer N-(3-oxododecanoyl) homoserine lactone in NCI-H292 cells. Antimicrob. Agents Chemother. 48(9): 3457-3461 CrossRef, Medline.Inoue D, Yamaya M, Kubo H, Sasaki T, Hosoda M, Numasaki M, et al.. 2006. Mechanisms of mucin production by rhinovirus infection in cultured human airway epithelial cells. Respir. Physiol. Neurobiol. 154(3): 484-499 CrossRef, Medline.Kraft M, Adler KB, Ingram JL, Crews AL, Atkinson TP, Cairns CB, et al.. 2008. Mycoplasma pneumoniae induces airway epithelial cell expression of MUC5AC in asthma. Eur. Respir. J. 31(1): 43-46 CrossRef, Medline.Lewinsohn DM, Alderson MR, Briden AL, Riddell SR, Reed SG, Grabstein KH. 1998. Characterization of human CD8+ T cells reactive with Mycobacterium tuberculosis-infected antigen-presenting cells. J. Exp. Med. 187(10): 1633-1640 CrossRef, Medline.Li D, Gallup M, Fan N, Szymkowski DE, Basbaum CB. 1998. Cloning of the amino-terminal and 5'-flanking region of the human MUC5AC mucin gene and transcriptional up-regulation by bacterial exoproducts. J. Biol. Chem. 273(12): 6812-6820 CrossRef, Medline.Lorenz J, Zahlten J, Pollok I, Lippmann J, Scharf S, N’Guessan PD, et al.. 2010. Legionella pneumophila induced i?b?-dependent expression of il-6 in lung epithelium. Eur. Respir. J. 37(3): 648-657 CrossRef, Medline.Morinaga Y, Yanagihara K, Miyashita N, Seki M, Izumikawa K, Kakeya H, et al.. 2009. Azithromycin, clarithromycin and telithromycin inhibit MUC5AC induction by Chlamydophila pneumoniae in airway epithelial cells. Pulm. Pharmacol. Ther. 22(6): 580-586 CrossRef, Medline.N’Guessan PD, Etouem MO, Schmeck B, Hocke AC, Scharf S, Vardarova K, et al.. 2007. Legionella pneumophila-induced PKCa-, MAPK-, and NF-?B-dependent COX-2 expression in human lung epithelium. Am. J. Physiol. Lung Cell. Mol. 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Physiol. 298(5): L687-L695 CrossRef, Medline.Schmeck B, N’Guessan PD, Ollomang M, Lorenz J, Zahlten J, Opitz B, et al.. 2007. Legionella pneumophila-induced NF-?B- and MAPK-dependent cytokine release by lung epithelial cells. Eur. Respir. J. 29(1): 25-33 CrossRef, Medline.Shao MX, Nadel JA. 2005. Neutrophil elastase induces MUC5AC mucin production in human airway epithelial cells via a cascade involving protein kinase C, reactive oxygen species, and TNF-a-converting enzyme. J. Immunol. 175(6): 4009-4016 Medline.Shao MX, Nakanaga T, Nadel JA. 2004. Cigarette smoke induces MUC5AC mucin overproduction via tumor necrosis factor-a-converting enzyme in human airway epithelial (NCI-H292) cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 287(2): L420-L427 CrossRef, Medline.Song KS, Lee WJ, Chung KC, Koo JS, Yang EJ, Choi JY, Yoon JH. 2003. Interleukin-1 ß and tumor necrosis factor-a induce MUC5AC overexpression through a mechanism involving ERK/p38 mitogen-activated protein kinases-MSK1-CREB activation in human airway epithelial cells. J. Biol. Chem. 278(26): 23243-23250 CrossRef, Medline.Teruya H, Higa F, Akamine M, Ishikawa C, Okudaira T, Tomimori K, et al.. 2007. Mechanisms of Legionella pneumophila-induced interleukin-8 expression in human lung epithelial cells. BMC Microbiol. 7(1): 102 CrossRef, Medline.Ünal C, Schwedhelm KF, Thiele A, Weiwad M, Schweimer K, Frese F, et al.. 2011. Collagen IV-derived peptide binds hydrophobic cavity of Legionella pneumophila Mip and interferes with bacterial epithelial transmigration. Cell. Microbiol. 13(10): 1558-1572 CrossRef, Medline.Wagner C, Khan AS, Kamphausen T, Schmausser B, Unal C, Lorenz U, et al.. 2007. Collagen binding protein Mip enables Legionella pneumophila to transmigrate through a barrier of NCI-H292 lung epithelial cells and extracellular matrix. Cell. Microbiol. 9(2): 450-462 CrossRef, Medline.Welsh CT, Summersgill JT, Miller RD. 2004. Increases in c-Jun N-terminal kinase/stress-activated protein kinase and p38 activity in monocyte-derived macrophages following the uptake of Legionella pneumophila. Infect. Immun. 72(3): 1512-1518 CrossRef, Medline.Williams OW, Sharafkhaneh A, Kim V, Dickey BF, Evans CM. 2006. Airway mucus: from production to secretion. Am. J. Respir. Cell Mol. Biol. 34(5): 527-536 CrossRef, Medline.Yanagihara K, Seki M, Cheng PW. 2001. Lipopolysaccharide induces mucus cell metaplasia in mouse lung. Am. J. Respir. Cell Mol. Biol. 24(1): 66-73 Medline.

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Correction for O'Connor et al., Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host range expansion [Correction]

MICROBIOLOGY Correction for “Minimization of the Legionella pneumophila genome reveals chromosomal regions involved in host …

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The htpAB operon of Legionella pneumophila cannot be deleted in the presence of the groE chaperonin operon of Escherichia coli

Gheyath K. Nasrallah,a Elizabeth Gagnon,a,* Dennis J. Orton,a,† Rafael A. Garduñoa,b aDepartment of Microbiology and Immunology, Dalhousie University, Sir Charles Tupper Medical Building, 7th Floor, 5850 College Street, Halifax, NS B3H 1X5, Canada.

bDepartment of Medicine — Division of Infectious Diseases, Dalhousie University, Dickson Building, 1276 South Park Street, Halifax, NS B3H 2Y9, Canada.

*Present address: Department of Microbiology and Immunology, The University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z4, Canada

†Present address: Department of Pathology, Dalhousie University, Sir Charles Tupper Medical Building, NS B3H 1X5, Canada

Published on the web 27 October 2011.

Canadian Journal of Microbiology, 2011, 57:(11) 943-952, 10.1139/w11-086

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Identification of Legionella pneumophila serogroups and other Legionella species by mip gene sequencing

Journal Article

PCR methods for the rapid detection and identification of four pathogenic Legionella spp. and two Legionella pneumophila subspecies based on the gene amplification of gyrB

Guangpeng Zhou, Boyang Cao, Yan Dou, Yanwei Liu and Lu Feng, et al.

Applied Microbiology and Biotechnology, 2011, Volume 91, Number 3, Pages 777-787

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Legionella pneumophila DNA in serum samples during Legionnaires’ disease in relation to C-reactive protein levels

F. L. van de Veerdonk, C. P. C. de Jager, J. J. A. Schellekens, C. J. J. Huijsmans and F. Beaumont, et al.

European Journal of Clinical Microbiology & Infectious Diseases, 2009, Volume 28, Number 4, Pages 371-376

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LAMP-based method for a rapid identification of Legionella spp. and Legionella pneumophila

Xi Lu, Zi-Yao Mo, Hong-Bo Zhao, He Yan and Lei Shi

Applied Microbiology and Biotechnology, 2011, Volume 92, Number 1, Pages 179-187

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Profiling of environmental Legionella pneumophila strains by randomly amplified polymorphic DNA method isolated from geographically nearby buildings

Zuhal Zeybek, Irfan Türetgen, Ayten Kimiran Erdem, Gönül Filoglu and Aysin Çotuk

Environmental Monitoring and Assessment, 2009, Volume 149, Numbers 1-4, Pages 323-327

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Anin vitro evaluation of the interactions ofLegionella pneumophila serogroups 2 to 14 strains with other bacteria in the same habitat

Ayten Kimiran Erdem and Aysegül Yazici

Annals of Microbiology, 2008, Volume 58, Number 3, Pages 395-401

Reference Work Entry

Legionella Species and Legionnaires’ Disease

Paul Edelstein and Nicholas Cianciotto

2006, The Prokaryotes, PART 3, SECTION 3.3, Pages 988-1033

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Screening-level assays for potentially human-infectious environmental Legionella spp.

Helen Y. Buse, Abby Brehm, Jorge W. Santo Domingo and Nicholas J. Ashbolt

The Journal of Microbiology, 2011, Volume 49, Number 2, Pages 200-207

Journal Article

Online First™ Efficacy of Colloidal Silver-Hydrogen Peroxide and 2-Bromo-2-nitroporopane-1,3-diol Compounds Against Different Serogroups of Legionella pneumophila Strains

N. O. Sanli-Yurudu, A. Kimiran-Erdem, E. O. Arslan-Aydogdu, Z. Zeybek and S. Gurun

Indian Journal of Microbiology, Online First™, 28 June 2011

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Non-chemotactic influence of CXCL7 on human phagocytes. Modulation of antimicrobial activity against L. pneumophila.

Authors: González-Cortés C, Diez-Tascón C, Guerra-Laso JM, González-Cocaño MC, Rivero-Lezcano OM Abstract We have investigated the role of CXCL7 in the immune response of human phagocytes against the intracellular bacteria Mycobacterium tuberculosis and Legionella pneumophila. We have observed that polymorphonuclear neutrophil (PMN) chemotaxis induced by the supernatants of infected monocyte derived macrophages (MDM) may be attributed to CXCL8 rather than CXCL7, although both chemokines are present in large quantities. We have also found that CXCL7 is present not only in the supernatants of MDM, but also in the supernatants of PMN of some, but not all, individuals. Western blot analysis revealed that, in both MDM and PMN supernatants appeared two bands with molecular weights c...

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Population variation in NAIP functional copy number confers increased cell death upon Legionella pneumophila infection.

Authors: Boniotto M, Tailleux L, Lomma M, Gicquel B, Buchrieser C, Garcia S, Quintana-Murci L Abstract The NAIP gene encodes an intracellular innate immunity receptor that senses flagellin. The genomic region containing NAIP presents a complex genomic organization and includes various NAIP paralogs. Here, we assessed the degree of copy number variation of the complete NAIP gene (NAIPFull) in various human populations and studied the functional impact of such variation on host cell fate using Legionella pneumophila as an infection model. We determined that African populations have a NAIPFull duplication at a higher frequency than Europeans and Asians, with an increased transcription of the gene. In addition, we demonstrated that a higher amount of the NAIPFull protein dramatically i...

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Legionella pneumophila urinary antigen subtyping using monoclonal antibodies as a tool for epidemiological investigations

Abstract  Legionnaires’ disease is diagnosed predominantly by urinary antigen detection, and patient isolates are rarely available. The lipopolysaccharide (LPS) epitope pattern of isolates detected by monoclonal antibodies is an accepted marker for the phenotyping of L. pneumophila serogroup 1 strains into monoclonal subgroups. L. pneumophila LPS is the dominant antigen in patients’ urinary specimens. By using commercially available microtiter wells coated with rabbit anti-Legionella serogroup 1 IgG as the catching antibody, LPS components in urine specimens were bound and detected separately by corresponding monoclonal antibodies of the Dresden Panel. The subtyping of LPS on urinary antigen molecules by using enzyme-linked immunosorbent assay (ELISA) allows deducing of ...

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The occurrence of Legionella species other than Legionella pneumophila in clinical and environmental samples in Denmark identified by mip gene sequencing and matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry

Contamination of the cold water distribution system of health care facilities by Legionella pneumophila: Do we know the true dimension?

(Source: Eurosurveillance latest news)

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Legionella pneumophila lung abscess associated with immune suppression

We describe Legionella pneumophila pneumonia and abscess formation in an immunosuppressed patient receiving corticosteroid therapy for metastatic breast carcinoma. The predisposing role of corticosteroids is discussed and the management of this complication is reviewed. (Source: Internal Medicine Journal)

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The immunochromatic kits Xpect(R) Legionella and BinaxNOW(R) Legionella for detection of Legionella pneumophila urinary antigen have low sensitivities for the diagnosis of Legionnaires' disease.

The occurrence of Legionella species other than L. pneumophila in clinical and environmental samples in Denmark identified by mip‐gene‐sequencing and MALDI‐TOF‐MS

AbstractIn Denmark, several laboratories use PCR as a routine diagnostic method for Legionnaires’ disease, and almost all PCR positive samples have been investigated by culture.From 1993-2010, we obtained isolates of Legionella species other than Legionella pneumophila (L. non- pneumophila) from respiratory samples from 33 patients, and from 1997-2010, 42 isolates of L. non- pneumophila were obtained and saved from water samples from 39 different sites in Denmark. mip-sequencing was used as a reference method to identify the L. non- pneumophila species. Only one of the 75 isolates did not pass the acceptance criteria with a similarity of = 98% to sequences in the database. The Species distribution between clinical and environmental isolates varied. For the former, four spec...

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Usefulness of real‐time PCR as a complementary tool to the monitoring of Legionella spp. and Legionella pneumophila by culture in industrial cooling systems

Conclusions: These results suggest that, if some methodological steps designed to reduce inhibitory problems and thus decrease the quantification limits, could be developed to quantify Legionella in complex waters, the real-time PCR could be a valuable complementary tool to monitor the evolution of L. pneumophila concentrations.Significance and Impact of the Study: This study shows the possibility of using real-time PCR to monitor L. pneumophila proliferations in cooling systems and the importance to adapt nucleic acid extraction and purification protocols to raw waters. (Source: Journal of Applied Microbiology)

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Diagnosis of Fulminant Pneumonia Caused by Legionella pneumophila Serogroup 8 with the Sequence Analysis of the 16S rRNA Gene.

In conclusion, this cultivation-independent method is a potential diagnostic modality for pneumonia, especially in patients with rapidly progressive pneumonia or those who are immunocompromised. PMID: 21878746 [PubMed - in process] (Source: The Tohoku Journal of Experimental Medicine)

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Covalent Coercion by Legionella pneumophila.

Authors: Itzen A, Goody RS Abstract Adenylylation of Rab proteins appears to be an intriguing mechanism that Legionella pneumophila uses to modulate their activity during infection. Now the reverse reaction (deadenylylation) (Neunuebel et al., 2011; Tan and Luo, 2011) and a new posttranslational modification (phosphocholination) of Rab1 (Mukherjee et al., 2011) have been reported. PMID: 21843863 [PubMed - in process] (Source: Cell Host and Microbe)

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Protein expression, crystallization and preliminary X-ray crystallographic studies of LidA from Legionella pneumophila

LidA, a translocated substrate of the Legionella pneumophila Dot/Icm type IV secretion system, is associated with maintenance of bacterial integrity and interferes with the early secretory pathway. However, the precise mechanism of LidA in these processes remains elusive. To further investigate the structure and function of LidA, the full-length protein was successfully expressed in Escherichia coli and purified. LidA was crystallized using sitting-drop vapour diffusion and diffracted to a resolution of 2.75 Å. The crystal belonged to space group P212121, with unit-cell parameters a = 57.5, b = 64.5, c = 167.3 Å, a = ß = ? = 90°. There is one molecule per asymmetric unit. (Source: Acta Crystallographica Section F)

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Contamination of the cold water distribution system of health care facilities by Legionella pneumophila: Do we know the true dimension?

(Source: Eurosurveillance latest news)

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Infliximab: Legionella pneumophila pneumonia: case report

(Source: Reactions)

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Contamination of the cold water distribution system of health care facilities by Legionella pneumophila: Do we know the true dimension?

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