Recovery of Legionella species from water samples using an internal method based on ISO 11731: suggestions for revision and implementation

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The occurrence of Legionella species other than Legionella pneumophila in clinical and environmental samples in Denmark identified by mip gene sequencing and matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry

Bacterial and fungal microflora in surgically removed lung cancer samples

Conclusions:A diversity of pathogens could be identified in surgically extracted tissue samples of patients with lung cancer, with mycoplasma strains being present in all samples. These results point to an etiologic role for chronic infection in lung carcinogenesis. Confirmation of these observations and additional studies are needed to further characterize the etiologic role of inflammation in lung carcinogenesis. (Source: Journal of Cardiothoracic Surgery)

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Recovery of Legionella species from water samples using an internal method based on ISO 11731: suggestions for revision and implementation

Abstract: The study aim was to determine retrospectively whether the parallel use of 2 media [buffered charcoal yeast extract (BCYE) and medium of Wadowsky and Yee (MWY)] to isolate Legionella spp. from water samples taken from hospital water supply systems increased the sensitivity of the culture method as compared with methods/protocols in which only seeding on a selective medium is used. We analyzed the results obtained from 931 positive water samples. In 484 of the 931 positive water samples, Legionella spp. was isolated in the presence of other microorganisms; in 83% (400/484), we used MWY to count suspected colonies, which gave a lower number of unreadable plates. In the 447 samples containing only Legionella spp., the highest frequency of positive samples (93%, 418/447) was obtained...

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The occurrence of Legionella species other than L. pneumophila in clinical and environmental samples in Denmark identified by mip‐gene‐sequencing and MALDI‐TOF‐MS

AbstractIn Denmark, several laboratories use PCR as a routine diagnostic method for Legionnaires’ disease, and almost all PCR positive samples have been investigated by culture.From 1993-2010, we obtained isolates of Legionella species other than Legionella pneumophila (L. non- pneumophila) from respiratory samples from 33 patients, and from 1997-2010, 42 isolates of L. non- pneumophila were obtained and saved from water samples from 39 different sites in Denmark. mip-sequencing was used as a reference method to identify the L. non- pneumophila species. Only one of the 75 isolates did not pass the acceptance criteria with a similarity of = 98% to sequences in the database. The Species distribution between clinical and environmental isolates varied. For the former, four spec...

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