Detection of microorganisms in exhaled breath condensate during acute exacerbations of COPD

Detection of microorganisms in exhaled breath condensate during acute exacerbations of COPD - Zakharkina - Respirology - Wiley Online LibrarySkip to Main Content

Home Help

PUBLICATIONSBROWSE BY SUBJECTRESOURCESABOUT US LOGIN Enter e-mail address Enter password REMEMBER ME NOT REGISTERED ?FORGOTTEN PASSWORD ?INSTITUTIONAL LOGIN > Home > Respiratory Medicine > Respiratory Medicine > Journal Home > Accepted Articles > Abstract JOURNAL TOOLS Get New Content Alerts Get RSS feed Save to My Profile Get Sample Copy Recommend to Your Librarian JOURNAL MENU Journal Home FIND ISSUES Current IssueAll IssuesVirtual Issues FIND ARTICLES Accepted Articles GET ACCESS Subscribe / Renew FOR CONTRIBUTORS Author GuidelinesSubmit an Article ABOUT THIS JOURNAL OverviewNewsEditorial BoardContactSociety InformationAdvertisePermissions Detection of microorganisms in exhaled breath condensate during acute exacerbations of COPDTetyana Zakharkina1,2,†, A.-Rembert Koczulla1,†, Olga Mardanova1, Akira Hattesohl1, Robert Bals1,2,*DOI: 10.1111/j.1440-1843.2011.01977.x

© 2011 The Authors. Respirology © 2011 Asian Pacific Society of Respirology

Issue

RespirologyAccepted Article (Accepted, unedited articles published online for future issues)

Additional Information

Author Information1

Department of Internal Medicine, Division for Pulmonary Diseases, University Hospital Giessen and Marburg, D-35043 Marburg, Germany

2

Department of Internal Medicine V -Pulmonology, Respiratory Intensive Care Medicine, Allergology, D-66421 Homburg, Germany

*Correspondence: Robert Bals,

*Correspondence: Prof. Dr. Dr. Robert Bals ?Department of Internal Medicine V -Pulmonology, Respiratory Intensive Care Medicine, Allergology ?D-66421 Homburg, Germany ?Tel: +49 (0)6841 16 23601 Fax: +49 (0) 6841 16 23602 ?E-mail: robert.bals@uks.eu

†

both authors contributed equally

This is an Accepted Article that has been peer-reviewed and approved for publication in the Respirology, but has yet to undergo copy-editing and proof correction. Please cite this article as an “Accepted Article”; doi: 10.1111/j.1440-1843.2011.01977.x

Publication HistoryAccepted manuscript online: 7 APR 2011 01:59AM ESTAccepted 21 February 2011 SEARCH Search Scope All contentPublication titlesIn this journalIn this issue Search String Advanced >Saved Searches > SEARCH BY CITATION Volume: Issue: Page: ARTICLE TOOLSGet PDF (194K)Save to My ProfileE-mail Link to this ArticleExport Citation for this ArticleGet Citation AlertsRequest Permissions AbstractCited By Get PDF (194K) Keywords:COPD;exacerbation;exhaled breath condensate;sputum;bacteria;virusesSummary at a Glance

Exhaled breath condensate (EBC) is a noninvasive method to assess airway biology. The aim of the present study was to evaluate EBC for the detection of microbial nucleic acids during acute exacerbations of COPD (AECOPD). Bacterial nucleic acids can be identified in EBCs of COPD patients with exacerbations, however, the results differed significantly from those of sputum.

ABSTRACT

Background and objective: One hallmark of chronic obstructive pulmonary disease (COPD) is colonization and infection of the lung. Acute exacerbations of COPD (AECOPD) are acute deteriorations of the chronic disease and are associated with a change of the pulmonary microbial balance. The collection of exhaled breath condensate (EBC) can be used to noninvasively determine markers of lung disease. The aim of the present study was to compare the results of assays based on the detection of microbial nucleic acids from exhaled breath condensate (EBC) and from spontaneous sputum in patients with AECOPD.

Methods: EBCs and sputa of 29 adults with AECOPD were obtained. Isolated DNA or RNA were used as starting material for the PCR assays to detect Staphylococcus aureus, Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae, Legionella pneumophila,Mycoplasma pneumoniae,Chlamydia pneumoniae, influenza viruses (AH 1, AH 3) and respiratory syncytial virus.

Results: Bacterial or viral nucleic acids were identified in 14 EBCs and 21 sputa from 29 patients. Results from EBC did not correlate well with those from sputum. Viral and S. pneumoniae nucleic acids were detected only in sputum, whereas L. pneumophila DNA was only found in EBC. In 3 EBCs and 10 sputa nucleic acids of more than one microorganism was detected.

Conclusions: Bacterial nucleic acids can be identified in EBCs of COPD patients with exacerbations. The results obtained from EBC and sputum did not correlate well.

Get PDF (194K) More content like this Find more content: like this article Find more content written by:Tetyana ZakharkinaA.-Rembert KoczullaOlga MardanovaAkira HattesohlRobert BalsAll Authors ABOUT USHELPCONTACT USAGENTSADVERTISERSMEDIAPRIVACYTERMS & CONDITIONSSITE MAP

Copyright © 1999–2011 John Wiley & Sons, Inc. All Rights Reserved.

View the original article here