AbstractIn Denmark, several laboratories use PCR as a routine diagnostic method for Legionnaires’ disease, and almost all PCR positive samples have been investigated by culture.From 1993-2010, we obtained isolates of Legionella species other than Legionella pneumophila (L. non- pneumophila) from respiratory samples from 33 patients, and from 1997-2010, 42 isolates of L. non- pneumophila were obtained and saved from water samples from 39 different sites in Denmark. mip-sequencing was used as a reference method to identify the L. non- pneumophila species. Only one of the 75 isolates did not pass the acceptance criteria with a similarity of = 98% to sequences in the database. The Species distribution between clinical and environmental isolates varied. For the former, four spec...
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