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Showing posts with label 11731. Show all posts
Showing posts with label 11731. Show all posts
Saturday, 10 December 2011
Recovery of Legionella species from water samples using an internal method based on ISO 11731: suggestions for revision and implementation
Friday, 25 November 2011
Recovery of Legionella species from water samples using an internal method based on ISO 11731: suggestions for revision and implementation
Abstract: The study aim was to determine retrospectively whether the parallel use of 2 media [buffered charcoal yeast extract (BCYE) and medium of Wadowsky and Yee (MWY)] to isolate Legionella spp. from water samples taken from hospital water supply systems increased the sensitivity of the culture method as compared with methods/protocols in which only seeding on a selective medium is used. We analyzed the results obtained from 931 positive water samples. In 484 of the 931 positive water samples, Legionella spp. was isolated in the presence of other microorganisms; in 83% (400/484), we used MWY to count suspected colonies, which gave a lower number of unreadable plates. In the 447 samples containing only Legionella spp., the highest frequency of positive samples (93%, 418/447) was obtained...
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