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Saturday, 3 December 2011
How Legionnaires' bacteria proliferate, cause disease
Scientist have determined for the first time how the bacterium that causes Legionnaires' disease manipulates our cells to generate the amino acids it needs to grow and cause infection and inflammation in the lungs. (Source: ScienceDaily Headlines)
Population variation in NAIP functional copy number confers increased cell death upon Legionella pneumophila infection.
Authors: Boniotto M, Tailleux L, Lomma M, Gicquel B, Buchrieser C, Garcia S, Quintana-Murci L Abstract The NAIP gene encodes an intracellular innate immunity receptor that senses flagellin. The genomic region containing NAIP presents a complex genomic organization and includes various NAIP paralogs. Here, we assessed the degree of copy number variation of the complete NAIP gene (NAIPFull) in various human populations and studied the functional impact of such variation on host cell fate using Legionella pneumophila as an infection model. We determined that African populations have a NAIPFull duplication at a higher frequency than Europeans and Asians, with an increased transcription of the gene. In addition, we demonstrated that a higher amount of the NAIPFull protein dramatically i...
Detection of Legionella by quantitative-Polymerase Chain Reaction (qPCR) for monitoring and risk assessment
Conclusion:Detection by qPCR was suitable for monitoring changes in the concentration of Legionella but the precise determination of bacteria is difficult. Risk assessment by qPCR only on samples without any background information regarding treatment, timing, etc is dubious. However, the rapid detection by qPCR of high concentrations of Legionella - especially Legionella pneumophila - is valuable as an indicator of risk, although it may be false positive compared to culture results. On the other hand, the detection of a low number of bacteria by qPCR is a strong indication for the absence of risk. (Source: BMC Microbiology - Latest articles)
How Legionnaires' bacteria cause disease
LOUISVILLE, Ky., Nov. 18 (UPI) -- A bacterium that causes Legionnaires' disease manipulates cells to generate amino acids it needs to grow and infect the lungs, U.S. researchers say. (Source: Health News - UPI.com)
Friday, 2 December 2011
UofL researcher determines how Legionnaires' bacteria proliferate, cause disease
(University of Louisville) A University of Louisville scientist has determined for the first time how the bacterium that causes Legionnaires' disease manipulates our cells to generate the amino acids it needs to grow and cause infection and inflammation in the lungs. The results are published online today (Nov. 17) in Science. (Source: EurekAlert! - Medicine and Health)
Legionella pneumophila urinary antigen subtyping using monoclonal antibodies as a tool for epidemiological investigations
Abstract Legionnaires’ disease is diagnosed predominantly by urinary antigen detection, and patient isolates are rarely available. The lipopolysaccharide (LPS) epitope pattern of isolates detected by monoclonal antibodies is an accepted marker for the phenotyping of L. pneumophila serogroup 1 strains into monoclonal subgroups. L. pneumophila LPS is the dominant antigen in patients’ urinary specimens. By using commercially available microtiter wells coated with rabbit anti-Legionella serogroup 1 IgG as the catching antibody, LPS components in urine specimens were bound and detected separately by corresponding monoclonal antibodies of the Dresden Panel. The subtyping of LPS on urinary antigen molecules by using enzyme-linked immunosorbent assay (ELISA) allows deducing of ...
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Researcher Determines How Legionnaires' Bacteria Proliferate, Cause Disease
A University of Louisville scientist has determined for the first time how the bacterium that causes Legionnaires' disease manipulates our cells to generate the amino acids it needs to grow and cause infection and inflammation in the lungs. The results are published online in Science. Yousef Abu Kwaik, Ph.D., the Bumgardner Endowed Professor in Molecular Pathogenesis of Microbial Infections at UofL, and his team believe their work could help lead to development of new antibiotics and vaccines... (Source: Health News from Medical News Today)
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